Review



progesterone release  (ALPCO)


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    Structured Review

    ALPCO progesterone release
    Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
    Progesterone Release, supplied by ALPCO, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/progesterone release/product/ALPCO
    Average 94 stars, based on 47 article reviews
    progesterone release - by Bioz Stars, 2026-05
    94/100 stars

    Images

    1) Product Images from "A surface-engineered microfluidic device for antibody-mediated negative selection of high-quality sperm for assisted reproduction"

    Article Title: A surface-engineered microfluidic device for antibody-mediated negative selection of high-quality sperm for assisted reproduction

    Journal: bioRxiv

    doi: 10.1101/2025.09.02.673619

    Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and progesterone coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
    Figure Legend Snippet: Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and progesterone coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.

    Techniques Used: Adsorption, Microscopy, Labeling, TUNEL Assay, Staining, Conjugation Assay



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    ALPCO progesterone release
    Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and <t>progesterone</t> coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.
    Progesterone Release, supplied by ALPCO, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/progesterone release/product/ALPCO
    Average 94 stars, based on 1 article reviews
    progesterone release - by Bioz Stars, 2026-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and progesterone coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.

    Journal: bioRxiv

    Article Title: A surface-engineered microfluidic device for antibody-mediated negative selection of high-quality sperm for assisted reproduction

    doi: 10.1101/2025.09.02.673619

    Figure Lengend Snippet: Fabrication of gold nano-particle-coated channel slides, containing areas of Anti-PS and progesterone coatings (the ‘NGS Device’) and its effect on immobilising low-quality sperm on the surface. (a) Surface coating with PPOx and addition of GNP to achieve covalent immobilization of COOH-GNP, followed by a second application of PPOx; covalent immobilization of anti-PS antibody (200 µg/mL) onto the glass surface next to the inlet well, and electrostatic adsorption of progesterone onto the half of the glass surface next to the outlet well. (b) Sperm attachment on the channel segment coated with Anti-PS. (c) The surface coated with progesterone exhibited a few or no sperm attachment. (d) Proportion of neat and attached sperm labelled by Annexin v was quantified by fluorescent microscopy. (e) The proportion of sperm labeled with TUNEL was quantified by fluorescent microscopy. (f) Representative fluorescent microscope images of neat samples stained with TUNEL (green) and DAI (blue). (g) Representative fluorescent microscope images of attached sperm on the neat and attached-sperm on Anti-PS-GNP coated surface stained with TUNEL (green) and DAPI (blue). (h, i) Bio-conjugation of Anti-PS using protein G and its effect on the reduction of apoptotic and necrotic sperm populations among recovered sperm through coated channel slides. One-way ANOVA followed by post-hoc t-test was used to evaluate differences between treatment groups. *P ≤ 0.01 and **P ≤ 0.001.

    Article Snippet: Progesterone release into the culture medium was quantified using a competitive Progesterone ELISA kit (ALPCO, Cat. No. 11-PROHU-E01), according to the manufacturer’s instructions.

    Techniques: Adsorption, Microscopy, Labeling, TUNEL Assay, Staining, Conjugation Assay